Topoisomerase IV Subunit B

(All numbering and residues are taken from first PDB file)

Bending Residue Dihedral Analysis

Residue i Residue i+1 Distance of hinge axis to residue i in conformer 1 (A) Distance of hinge axis to residue i in conformer 2 (A) Change in psi (i) (deg) Change in phi (i+1) (deg) Angle of psi(i) axis to hinge axis conformer 1 (deg) Angle of psi(i) axis to hinge axis conformer 2 (deg) Percentage Progress  CYS-1214   TYR-1215  10.3 10.1 17.1 -14.6 114.0 117.1 16.7  TYR-1215   GLN-1216  10.0 10.1 -31.5 33.8 16.4 29.5 -5.6  GLN-1216   ASP-1217  11.9 12.8 -3.3 -3.3 57.4 32.9 43.3  ASP-1217   GLY-1218  10.9 11.4 98.1 -54.8 73.6 91.5 59.5  GLY-1218   LEU-1219  7.9 7.8 -19.8 -10.7 93.3 104.2 21.7  LEU-1219   ASN-1220  7.2 7.2 13.9 -14.1 71.0 73.6 -1.9 Graph shows rotational transition at bending residues and can be used to identify hinge bending residues. Probably only informative for interdomain rotations greater than 20 degrees