Pyruvate Kinase

(All numbering and residues are taken from first PDB file)

Bending Residue Dihedral Analysis

Residue i Residue i+1 Distance of hinge axis to residue i in conformer 1 (A) Distance of hinge axis to residue i in conformer 2 (A) Change in psi (i) (deg) Change in phi (i+1) (deg) Angle of psi(i) axis to hinge axis conformer 1 (deg) Angle of psi(i) axis to hinge axis conformer 2 (deg) Percentage Progress  GLY-91   PRO-92  8.6 8.6 3.4 -5.2 101.1 100.1 -18.7  PRO-92   GLU-93  7.9 7.9 -84.2 71.2 17.5 22.7 181.4  GLU-93   ILE-94  9.1 9.0 -16.5 12.4 90.1 90.8 -41.0 Graph shows rotational transition at bending residues and can be used to identify hinge bending residues. Probably only informative for interdomain rotations greater than 20 degrees

Residue i Residue i+1 Distance of hinge axis to residue i in conformer 1 (A) Distance of hinge axis to residue i in conformer 2 (A) Change in psi (i) (deg) Change in phi (i+1) (deg) Angle of psi(i) axis to hinge axis conformer 1 (deg) Angle of psi(i) axis to hinge axis conformer 2 (deg) Percentage Progress  PRO-189   ASN-190  8.5 8.3 6.0 -3.3 138.5 135.2 33.0  ASN-190   THR-191  9.8 9.7 2.1 6.1 130.5 128.1 -149.5  THR-191   LYS-192  9.7 9.8 -2.1 0.2 48.1 44.4 106.6  LYS-192   ILE-193  7.9 8.3 -1.0 2.5 74.2 73.2 -54.7  ILE-193   PRO-194  8.8 9.2 2.1 -2.4 133.6 133.7 20.1  PRO-194   PHE-195  7.9 8.2 -0.7 3.6 96.0 95.6 -29.1  PHE-195   PRO-196  7.3 7.5 0.1 -9.2 142.0 144.8 118.4  PRO-196   ALA-197  7.2 7.1 -0.3 10.1 104.5 104.0 -2.1 Graph shows rotational transition at bending residues and can be used to identify hinge bending residues. Probably only informative for interdomain rotations greater than 20 degrees

Residue i Residue i+1 Distance of hinge axis to residue i in conformer 1 (A) Distance of hinge axis to residue i in conformer 2 (A) Change in psi (i) (deg) Change in phi (i+1) (deg) Angle of psi(i) axis to hinge axis conformer 1 (deg) Angle of psi(i) axis to hinge axis conformer 2 (deg) Percentage Progress  GLU-250   ASN-251  3.3 3.4 28.1 0.0 88.6 77.3 -171.4  ASN-251   GLN-252  4.1 3.7 3.3 -2.0 57.9 62.8 -2.0  GLN-252   GLN-253  7.6 7.2 1.1 -3.1 12.7 18.5 16.4  GLN-253   GLY-254  6.3 6.1 0.4 7.4 82.5 86.8 13.1  GLY-254   ILE-255  5.6 5.8 -1.5 -3.1 80.6 77.3 -43.7  ILE-255   ASP-256  9.1 9.1 -1.2 -0.8 138.7 141.5 30.3 Graph shows rotational transition at bending residues and can be used to identify hinge bending residues. Probably only informative for interdomain rotations greater than 20 degrees