Pyruvate Kinase

(All numbering and residues are taken from first PDB file)

Bending Residue Dihedral Analysis

Residue i Residue i+1 Distance of hinge axis to residue i in conformer 1 (A) Distance of hinge axis to residue i in conformer 2 (A) Change in psi (i) (deg) Change in phi (i+1) (deg) Angle of psi(i) axis to hinge axis conformer 1 (deg) Angle of psi(i) axis to hinge axis conformer 2 (deg) Percentage Progress  GLY-86   PRO-87  5.4 5.4 -15.3 1.9 93.1 86.6 27.6  PRO-87   GLU-88  1.6 2.3 61.8 -12.5 33.3 36.3 119.6  GLU-88   ILE-89  3.6 4.3 14.4 -14.1 97.6 93.7 -13.5 Graph shows rotational transition at bending residues and can be used to identify hinge bending residues. Probably only informative for interdomain rotations greater than 20 degrees

Residue i Residue i+1 Distance of hinge axis to residue i in conformer 1 (A) Distance of hinge axis to residue i in conformer 2 (A) Change in psi (i) (deg) Change in phi (i+1) (deg) Angle of psi(i) axis to hinge axis conformer 1 (deg) Angle of psi(i) axis to hinge axis conformer 2 (deg) Percentage Progress  LEU-186   PRO-187  3.4 4.6 19.2 11.3 64.2 59.7 7.3  PRO-187   ALA-188  2.9 3.4 -21.7 2.6 66.4 58.0 17.5  ALA-188   VAL-189  5.3 5.3 35.9 -11.2 78.8 88.9 32.0 Graph shows rotational transition at bending residues and can be used to identify hinge bending residues. Probably only informative for interdomain rotations greater than 20 degrees